验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, hTERT-RPE1 cells, human lung tissue |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66235-1-Ig targets IL-33 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag21430 Product name: Recombinant human IL-33 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-270 aa of BC047085 Sequence: MKPKMKYSTNKISTAKWKNTASKALCFKLGKSQQKAKEVCPMYFMKLRSGLMIKKEACYFRRETTKRPSLKTGRKHKRHLVLAACQQQSTVECFAFGISGVQKYTRALHDSSITGISPITEYLASLSTYNDQSITFALEDESYEIYVEDLKKDEKKDKVLLSYYESQHPSNESGDGVDGKMLMVTLSPTKDFWLHANNKEHSVELHKCEKPLPDQAFFVLHNMHSNCVSFECKTDPGVFIGVKDNHLALIKVDSSENLCTENILFKLSET 种属同源性预测 |
| 宿主/亚型 | Mouse / IgM |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 33 |
| 别名 | C9orf26, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2 |
| 计算分子量 | 30 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC047085 |
| 基因名称 | IL-33 |
| Gene ID (NCBI) | 90865 |
| RRID | AB_2881624 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Thiophilic affinity chromatograph |
| UNIPROT ID | O95760 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IL-33 is an IL-1 family cytokine and nuclear alarmin, and is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for IL-33 antibody 66235-1-Ig | Download protocol |
| WB protocol for IL-33 antibody 66235-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Oncol Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression. | ||
Cell Cycle Roles of Clara cell 10-kD protein and type 2 innate lymphoid cells in allergic rhinitis. | ||
Sci Rep Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression. | ||
Cells Helicobacter pylori Induces IL-33 Production and Recruits ST-2 to Lipid Rafts to Exacerbate Inflammation. | ||
Int J Mol Sci An Amide Alkaloid Isolated from Ephedra sinica Ameliorates OVA-Induced Allergic Asthma by Inhibiting Mast Cell Activation and Dendritic Cell Maturation | ||
Cell Death Dis Macrophages originated IL-33/ST2 inhibits ferroptosis in endometriosis via the ATF3/SLC7A11 axis |
