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验证数据展示

  • WB analysis of Recombinant protein using 60290-1-Ig

    WB analysis of Recombinant protein using 60290-1-Ig

    Recombinant protein were subjected to SDS PAGE followed by western blot with 60290-1-Ig (IL1F8 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 60290-1-Ig

    IHC staining of human tonsillitis using 60290-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60290-1-Ig (IL36 beta/IL1F8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 60290-1-Ig

    IHC staining of human tonsillitis using 60290-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60290-1-Ig (IL36 beta/IL1F8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 60290-1-Ig

    IF Staining of human tonsillitis using 60290-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-36 Beta/IL-1F8 antibody (60290-1-Ig, Clone: 10D12B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 60290-1-Ig

    IF Staining of human tonsillitis using 60290-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-36 Beta/IL-1F8 antibody (60290-1-Ig, Clone: 10D12B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

IL-36 Beta/IL-1F8 Monoclonal antibody

IL-36 Beta/IL-1F8 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60290-1-Ig

产品说明书

CloneNo. 10D12B1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

IL1F8, 10D12B1, IL 1F8, IL 1H2, IL-1 eta

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRecombinant protein
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60290-1-Ig targets IL-36 Beta/IL-1F8 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12675

Product name: Recombinant human IL-1F8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-157 aa of BC101833

Sequence: MNPQREAAPKSYAIRDSRQMVWVLSGNSLIAAPLSRSIKPVTLHLIACRDTEFSDKEKGNMVYLGIKGKDLCLFCAEIQGKPTLQLKEKNIMDLYVEKKAQKPFLFFHNKEGSTSVFQSVSYPGWFIATSTTSGQPIFLTKERGITNNTNFYLDSVE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 1 family, member 8 (eta)
别名 IL1F8, 10D12B1, IL 1F8, IL 1H2, IL-1 eta
计算分子量 157 aa, 18 kDa
观测分子量 44 kDa
GenBank蛋白编号BC101833
基因名称 IL-36 Beta/IL-1F8
Gene ID (NCBI) 27177
RRIDAB_2881406
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9NZH7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for IL-36 Beta/IL-1F8 antibody 60290-1-IgDownload protocol
IHC protocol for IL-36 Beta/IL-1F8 antibody 60290-1-IgDownload protocol
WB protocol for IL-36 Beta/IL-1F8 antibody 60290-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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