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验证数据展示

  • WB analysis of Raji using 60138-1-Ig

    WB analysis of Raji using 60138-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 60138-1-Ig (IL15RA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IL-15RA Monoclonal antibody

IL-15RA Monoclonal Antibody for WB, ELISA
Cat No. 60138-1-Ig

产品说明书

CloneNo. 5E3E1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, ELISA

别名

IL15RA, 5E3E1, CD215, IL 15 receptor subunit alpha, IL 15R alpha

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, Daudi cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60138-1-Ig targets IL-15RA in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 IL-15RA fusion protein Ag10200 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 15 receptor, alpha
别名 IL15RA, 5E3E1, CD215, IL 15 receptor subunit alpha, IL 15R alpha
计算分子量 231 aa, 24 kDa
观测分子量 55 kDa
GenBank蛋白编号BC107777
基因名称 IL-15RA
Gene ID (NCBI) 3601
RRIDAB_2881352
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13261
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897). The calculated molecular weight of full-length IL-15Rα is 28 kDa, IL-15Rα can be glycosylated, and larger apparent molecular weights of 55-65 kDa have been reported by some researches (PMID: 10480910; 15976182; 15671076; 17912462).

实验方案

Product Specific Protocols
WB protocol for IL-15RA antibody 60138-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols