• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of Transfected HEK-293T using CL647-98255

    FC experiment of Transfected HEK-293T using CL647-98255

    1x10^6 untransfected or IL-4 transfected HEK-293T cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Rat IL-4 Rabbit RecAb (CL647-98255, Clone: 241249H7). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of Transfected HEK-293T using CL647-98255

    FC experiment of Transfected HEK-293T using CL647-98255

    1x10^6 untransfected or IL-4 transfected HEK-293T cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Rat IL-4 Rabbit RecAb (CL647-98255, Clone: 241249H7) (red) or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647 Anti-Rat IL-4 Rabbit Recombinant Antibody

IL-4 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. CL647-98255

产品说明书

CloneNo. 241249H7

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

FC (Intra)

别名

241249H7, B-cell growth factor 1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BSF-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC (Intra) detected inTransfected HEK-293T cells
For other applications, we recommend the unconjugated version of this antibody, 98255-1-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-98255 targets IL-4 in FC (Intra) applications and shows reactivity with rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 rat
免疫原 IL-4 fusion protein Eg1113 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 4
别名 241249H7, B-cell growth factor 1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BSF-1
计算分子量16kDa
GenBank蛋白编号NM_201270.1
基因名称 IL-4
Gene ID (NCBI) 287287
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20096
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

IL-4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 IL-4 antibody CL647-98255Download protocol
Standard Protocols
Click here to view our Standard Protocols