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验证数据展示

  • FC experiment of human PBMCs using 98363-1-RR (same clone as 98363-1-PBS)

    FC experiment of human PBMCs using 98363-1-RR (same clone as 98363-1-PBS)

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-3 Rabbit RecAb (98363-1-RR, Clone: 240417E1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. Treated human PBMCs were pre-stimulated with anti-human CD3/CD28 and recombinant human IL-2 and IL-4. This data was developed using the same antibody clone with 98363-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98363-1-RR (same clone as 98363-1-PBS)

    FC experiment of human PBMCs using 98363-1-RR (same clone as 98363-1-PBS)

    1x10^6 PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-3 Rabbit RecAb (98363-1-RR, Clone: 240417E1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-3 Rabbit RecAb (98363-1-RR, Clone: 240417E1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. Treated human PBMCs were pre-stimulated with anti-human CD3/CD28 and recombinant human IL-2 and IL-4. This data was developed using the same antibody clone with 98363-1-PBS in a different storage buffer formulation.

Anti-Human IL-3 Rabbit Recombinant Antibody, PBS Only

IL-3 Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 98363-1-PBS

产品说明书

CloneNo. 240417E1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

IL3, IL 3, Interleukin 3, Interleukin-3, Multipotential colony-stimulating factor

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98363-1-PBS targets IL-3 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Eg0829

Product name: Recombinant Human IL-3 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 20-152 aa of NM_000588.3

Sequence: APMTQTTPLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 3 (colony-stimulating factor, multiple)
别名 IL3, IL 3, Interleukin 3, Interleukin-3, Multipotential colony-stimulating factor
计算分子量17 kDa
GenBank蛋白编号NM_000588.3
基因名称 IL-3
Gene ID (NCBI) 3562
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08700
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. (PMID: 24103757;2698876;2809196)

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