验证数据展示
经过测试的应用
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | Transfected CHO cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98735-3-RR targets IL-24 in IHC, FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | IHC, FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg4971 Product name: Recombinant Human IL-24 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 52-206 aa of BC009681 Sequence: QEFHFGPCQVKGVVPQKLWEAFWAVKDTMQAQDNITSARLLQQEVLQNVSDAESCYLVHTLLEFYLKTVFKNYHNRTVEVRTLKSFSTLANNFVLIVSQLQPSQENEMFSIRDSAHRRFLLFRRAFKQLDVEAALTKALGEVDILLTWMQKFYKL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interleukin 24 |
| 别名 | IL24, IL 24, interleukin 24, Interleukin-24, Melanoma differentiation-associated gene 7 protein |
| 计算分子量 | 207 aa, 24 kDa |
| GenBank蛋白编号 | BC009681 |
| 基因名称 | IL-24 |
| Gene ID (NCBI) | 11009 |
| RRID | AB_3746304 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13007 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
Interleukin-24 (IL-24), also known as Melanoma Differentiation-Associated gene 7 (mda-7), is a protein that belongs to the IL-10 family of cytokines. It was initially discovered in 1995 by researchers Paul B. Fisher and colleagues as a gene that is induced during the terminal differentiation of melanoma cells. IL-24 is secreted by multiple cell types, such as immune cells (T cells, B cells, NK cells, macrophages), and non-immune cells (epithelial cells and fibroblasts).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for IL-24 antibody 98735-3-RR | Download protocol |
| IHC protocol for IL-24 antibody 98735-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
