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验证数据展示

  • FC experiment of CHO using 98735-3-RR (same clone as 98735-3-PBS)

    FC experiment of CHO using 98735-3-RR (same clone as 98735-3-PBS)

    1x10^6 CHO cells or IL-24 transfected CHO cells were intracellularly stained with 0.25 ug Anti-Human IL-24 Rabbit RecAb (98735-3-RR, Clone: 251947A2) and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98735-3-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 98735-3-RR (same clone as 98735-3-PBS)

    IHC staining of human tonsillitis using 98735-3-RR (same clone as 98735-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98735-3-RR (IL-24 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98735-3-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 98735-3-RR (same clone as 98735-3-PBS)

    IHC staining of human tonsillitis using 98735-3-RR (same clone as 98735-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98735-3-RR (IL-24 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98735-3-PBS in a different storage buffer formulation.

  • Affinity of 98735-3-RR

    Affinity of 98735-3-RR

    Biolayer interferometry (BLI) kinetic assays of 98735-3-RR against Human IL-24 were performed. The affinity constant is below 1 pM.

Anti-Human IL-24 Rabbit Recombinant Antibody, PBS Only

IL-24 Uni-rAb® Recombinant Antibody for IHC, FC (Intra)
Cat No. 98735-3-PBS

产品说明书

CloneNo. 251947A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC (Intra)

别名

IL24, IL 24, interleukin 24, Interleukin-24, Melanoma differentiation-associated gene 7 protein

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98735-3-PBS targets IL-24 in IHC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IHC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Eg4971

Product name: Recombinant Human IL-24 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 52-206 aa of BC009681

Sequence: QEFHFGPCQVKGVVPQKLWEAFWAVKDTMQAQDNITSARLLQQEVLQNVSDAESCYLVHTLLEFYLKTVFKNYHNRTVEVRTLKSFSTLANNFVLIVSQLQPSQENEMFSIRDSAHRRFLLFRRAFKQLDVEAALTKALGEVDILLTWMQKFYKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 24
别名 IL24, IL 24, interleukin 24, Interleukin-24, Melanoma differentiation-associated gene 7 protein
计算分子量 207 aa, 24 kDa
GenBank蛋白编号BC009681
基因名称 IL-24
Gene ID (NCBI) 11009
RRIDAB_3746304
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13007
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-24 (IL-24), also known as Melanoma Differentiation-Associated gene 7 (mda-7), is a protein that belongs to the IL-10 family of cytokines. It was initially discovered in 1995 by researchers Paul B. Fisher and colleagues as a gene that is induced during the terminal differentiation of melanoma cells. IL-24 is secreted by multiple cell types, such as immune cells (T cells, B cells, NK cells, macrophages), and non-immune cells (epithelial cells and fibroblasts).

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