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验证数据展示

  • FC experiment of mouse splenocytes using FITC-98055

    FC experiment of mouse splenocytes using FITC-98055

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated mouse splenocytes were intracellularly stained with CoraLite® Plus 647 Anti-Mouse CD4 and 0.25 ug FITC Plus Anti-Mouse IL-2 Rabbit RecAb (FITC-98055, Clone: 240358A2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of mouse splenocytes using FITC-98055

    FC experiment of mouse splenocytes using FITC-98055

    1x10^6 PMA, Ionomycin and Brefeldin A treated mouse splenocytes were intracellularly stained with 0.25 ug FITC Plus Anti-Mouse IL-2 Rabbit RecAb (FITC-98055, Clone: 240358A2) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). 1x10^6 untreated mouse splenocytes were intracellularly stained with 0.25 ug FITC Plus Anti-Mouse IL-2 Rabbit RecAb (FITC-98055, Clone: 240358A2) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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FITC Plus Anti-Mouse IL-2 Rabbit Recombinant Antibody

IL-2 Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. FITC-98055

产品说明书

CloneNo. 240358A2

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC (Intra)

别名

Il2, 240358A2, Il 2, interleukin 2, Interleukin-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  FITC Plus
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inPMA, Ionomycin and Brefeldin A treated mouse splenocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-98055 targets IL-2 in FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 mouse
免疫原

CatNo: Eg0762

Product name: Recombinant Mouse IL-2 protein (His Tag)

Source: mammalian cells-derived, pCMV (S9)

Tag: N-6*His

Domain: 21-169 aa of NM_008366

Sequence: APTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 2
别名 Il2, 240358A2, Il 2, interleukin 2, Interleukin-2
计算分子量 19 kDa
GenBank蛋白编号 NM_008366
基因名称 IL-2
Gene ID (NCBI) 16183
偶联类型 FITC Plus Fluorescent Dye
最大激发/发射波长495 nm / 524 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP04351
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).

实验方案

Product Specific Protocols
FC protocol for FITC Plus IL-2 antibody FITC-98055Download protocol
Standard Protocols
Click here to view our Standard Protocols