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验证数据展示

  • WB analysis using 87348-2-RR (same clone as 87348-2-PBS)

    WB analysis using 87348-2-RR (same clone as 87348-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87348-2-RR (IL-18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87348-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse spleen using 87348-2-RR (same clone as 87348-2-PBS)

    IP experiment of mouse spleen using 87348-2-RR (same clone as 87348-2-PBS)

    IP result of anti-IL-18 (IP:87348-2-RR, 4ug; Detection:87348-2-RR 1:1500) with mouse spleen tissue lysate 2000 ug. This data was developed using the same antibody clone with 87348-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 87348-2-RR (same clone as 87348-2-PBS)

    IHC staining of mouse spleen using 87348-2-RR (same clone as 87348-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87348-2-RR (IL-18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87348-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 87348-2-RR

    Affinity and Kinetic Characterization of 87348-2-RR

    Biolayer interferometry (BLl) kinetic assays of 87348-2-RR against Mouse IL-18 were performed. The affinity constant is 2.55 nM.

Mouse IL-18 Recombinant monoclonal antibody, PBS Only

IL-18 Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 87348-2-PBS

产品说明书

CloneNo. 252154A6

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

IFN-gamma-inducing factor, Igif, IL-1 gamma, Il18, Interferon gamma-inducing factor

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87348-2-PBS targets IL-18 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg4455

Product name: Recombinant Mouse IL-18 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 1-192 aa of NM_008360.1

Sequence: MAAMSEDSCVNFKEMMFIDNTLYFIPEENGDLESDNFGRLHCTTAVIRNINDQVLFVDKRQPVFEDMTDIDQSASEPQTRLIIYMYKDSEVRGLAVTLSVKDSKMSTLSCKNKIISFEEMDPPENIDDIQSDLIFFQKRVPGHNKMEFESSLYEGHFLACQKEDDAFKLILKKKDENGDKSVMFTLTNLHQS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 18
别名 IFN-gamma-inducing factor, Igif, IL-1 gamma, Il18, Interferon gamma-inducing factor
计算分子量22 kDa
观测分子量22 kDa
GenBank蛋白编号NM_008360.1
基因名称 Il18
Gene ID (NCBI) 16173
RRIDAB_3745483
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP70380
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IL-18 is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma, which plays an important role in activating the macrophages or other cells. IL-18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL-18 is synthesized as a 22 kDa precursor and then cleaved into a biologically active 18 kDa form. This antibody recognizes both the pre-IL18 and mature IL18 theoretically.

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