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CoraLite® Plus 488-conjugated IL-17D Monoclonal antibody

IL-17D Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D7H4

Cat No : CL488-66143

Print datasheet

Synonyms

FLJ30846, IL 17D, IL 27, IL17D, IL27, interleukin 17D, Interleukin 27

验证数据展示

  • FC experiment of Jurkat using CL488-66143

    FC experiment of Jurkat using CL488-66143

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IL-17D (CL488-66143, Clone:1D7H4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66143-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66143 targets IL-17D in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra)
Tested Reactivity human
Immunogen IL-17D fusion protein Ag6762 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name interleukin 17D
Synonyms FLJ30846, IL 17D, IL 27, IL17D, IL27, interleukin 17D, Interleukin 27
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC036243
Gene Symbol IL17D
Gene ID (NCBI) 53342
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TAD2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL17D is a secreted cytokine with homology to the IL-17 family of proteins. IL17D is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas. The treatment of endothelial cells with IL17D has been shown to stimulate the production of other cytokines including IL6, IL8 and CSF2/ GM-CSF. The increased expression of IL8 induced by IL17D was found to be NF-kappa B-dependent.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IL-17D antibody CL488-66143Download protocol
Standard Protocols
Click here to view our Standard Protocols