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CoraLite® Plus 488-conjugated IL-12/IL-23 p40 Monoclonal antibody

IL-12/IL-23 p40 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4B2A8

Cat No : CL488-66288

Print datasheet

Synonyms

CLMF, CLMF p40, CLMF2, IL 12 subunit p40, IL 12B, IL-12/IL-23 p40, IL12B, Interleukin 12 subunit beta, NKSF, NKSF2

验证数据展示

  • FC experiment of Jurkat using CL488-66288

    FC experiment of Jurkat using CL488-66288

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IL-12B (CL488-66288, Clone:4B2A8) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66288-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66288 targets IL-12/IL-23 p40 in FC (Intra) applications and shows reactivity with human, pig samples.

Tested Applications FC (Intra)
Tested Reactivity human, pig
Immunogen IL-12/IL-23 p40 fusion protein Ag21429 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Synonyms CLMF, CLMF p40, CLMF2, IL 12 subunit p40, IL 12B, IL-12/IL-23 p40, IL12B, Interleukin 12 subunit beta, NKSF, NKSF2
Calculated Molecular Weight 328 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC067498
Gene Symbol IL12B
Gene ID (NCBI) 3593
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29460
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IL-12/IL-23 p40 antibody CL488-66288Download protocol
Standard Protocols
Click here to view our Standard Protocols