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IL-12/IL-23 p40 Monoclonal antibody

IL-12/IL-23 p40 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4B2A8

Cat No : 66288-1-Ig

Print datasheet

Synonyms

CLMF, CLMF p40, CLMF2, IL 12 subunit p40, IL 12B, IL12B, IL-12B, Interleukin 12 subunit beta, NKSF, NKSF2

验证数据展示

  • WB analysis of human liver using 66288-1-Ig

    WB analysis of human liver using 66288-1-Ig

    human liver tissue were subjected to SDS PAGE followed by western blot with 66288-1-Ig (IL12B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman liver tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66288-1-Ig targets IL-12/IL-23 p40 in WB, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, ELISA
Tested Reactivity human, pig
Immunogen IL-12/IL-23 p40 fusion protein Ag21429 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Synonyms CLMF, CLMF p40, CLMF2, IL 12 subunit p40, IL 12B, IL12B, IL-12B, Interleukin 12 subunit beta, NKSF, NKSF2
Calculated Molecular Weight 328 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC067498
Gene Symbol IL12B
Gene ID (NCBI) 3593
RRIDAB_2881671
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29460
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

实验方案

Product Specific Protocols
WB protocol for IL-12/IL-23 p40 antibody 66288-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols