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验证数据展示

  • FC experiment of human PBMCs using 98277-1-RR (same clone as 98277-1-PBS)

    FC experiment of human PBMCs using 98277-1-RR (same clone as 98277-1-PBS)

    1x10^6 untreated or IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-12/IL-23 p40 Rabbit Recombinant Antibody (98277-1-RR, Clone: 241600F8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98277-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98277-1-RR (same clone as 98277-1-PBS)

    FC experiment of human PBMCs using 98277-1-RR (same clone as 98277-1-PBS)

    1x10^6 IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL12B Rabbit Recombinant Antibody (98277-1-RR, Clone: 241600F8) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were stained with 0.25 ug Anti-Human IL-12/IL-23 p40 Rabbit Recombinant Antibody (98277-1-RR, Clone: 241600F8) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98277-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98277-1-RR

    Affinity and Kinetic Characterization of 98277-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98277-1-RR against Human IL-12/IL-23 p40 were performed. The affinity constant is below 1 pM.

Anti-Human IL-12/IL-23 p40 Rabbit Recombinant Antibody, PBS Only

IL-12/IL-23 p40 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98277-1-PBS

产品说明书

CloneNo. 241600F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

IL12B, 241600F8, CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL 12 P40

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

98277-1-PBS targets IL-12/IL-23 p40 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
别名 IL12B, 241600F8, CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL 12 P40
计算分子量 328 aa, 37 kDa
GenBank蛋白编号BC067498
基因名称 IL12B
Gene ID (NCBI) 3593
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP29460
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).