验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21465-1-AP targets IGSF21 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag15062 Product name: Recombinant human IGSF21 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 117-467 aa of BC007618 Sequence: HVGIYDRATREKVVLASGNIFLNVMAPPTSIEVVAADTPAPFSRYQAQNFTLVCIVSGGKPAPMVYFKRDGEPIDAVPLSEPPAASSGPLQDSRPFRSLLHRDLDDTKMQKSLSLLDAENRGGRPYTERPSRGLTPDPNILLQPTTENIPETVVSREFPRWVHSAEPTYFLRHSRTPSSDGTVEVRALLTWTLNPQIDNEALFSCEVKHPALSMPMQAEVTLVAPKGPKIVMTPSRARVGDTVRILVHGFQNEVFPEPMFTWTRVGSRLLDGSAEFDGKELVLERVPAELNGSMYRCTAQNPLGSTDTHTRLIVFENPNIPRGTEDSNGSIGPTGARLTLVLALTVILELT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobin superfamily, member 21 |
| 别名 | |
| 计算分子量 | 467 aa, 52 kDa |
| 观测分子量 | 48-50 kDa |
| GenBank蛋白编号 | BC007618 |
| 基因名称 | IGSF21 |
| Gene ID (NCBI) | 84966 |
| RRID | AB_10734429 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96ID5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IGSF21 (Immunoglobulin superfamily member 21) is a GPI-anchored protein that contains two immunoglobulin-like domains (Ig-like 1 and Ig-like 2). The Ig-like 1 domain of IGSF21 is indispensable for synaptogenic activity whereas its Ig-like 2 domain is secondarily responsible for the activity. It has been reported that IGSF21 selectively regulates inhibitory presynaptic differentiation through interacting with presynaptic neurexin2α and plays a crucial role in synaptic inhibition in the brain. (PMID: 28864826)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IGSF21 antibody 21465-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2α.
| ||
EMBO Rep Schizophrenia-related Xpo7 haploinsufficiency leads to behavioral and nuclear transport pathologies | ||
Front Immunol IgLON5 deficiency produces behavioral alterations in a knockout mouse model | ||
Front Mol Neurosci Structural and functional characterization of the IgSF21-neurexin2α complex and its related signaling pathways in the regulation of inhibitory synapse organization |