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验证数据展示

  • WB analysis using 86939-1-RR (same clone as 86939-1-PBS)

    WB analysis using 86939-1-RR (same clone as 86939-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86939-1-RR (IGJ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86939-1-PBS in a different storage buffer formulation.

  • IF Staining of Ramos using 86939-1-RR (same clone as 86939-1-PBS)

    IF Staining of Ramos using 86939-1-RR (same clone as 86939-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Ramos cells using IGJ antibody (86939-1-RR, Clone: 251691G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86939-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86939-1-RR

    Affinity and Kinetic Characterization of 86939-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86939-1-RR against Human IGJ were performed. The affinity constant is below 1 pM.

IGJ Recombinant monoclonal antibody, PBS Only

IGJ Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86939-1-PBS

产品说明书

CloneNo. 251691G5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

IGCJ, Immunoglobulin J chain, JCH, JCHAIN, Joining chain of multimeric IgA and IgM

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IGJ Ready-To-Use IHC Kit. IGJ primary antibody included.

产品信息

86939-1-PBS targets IGJ in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4559

Product name: Recombinant human IGJ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-159 aa of BC038982

Sequence: MKNHLLFWGVLAVFIKAVHVKAQEDERIVLVDNKCKCARITSRIIRSSEDPNEDIVERNIRIIVPLNNRENISDPTSPLRTRFVYHLSDLCKKCDPTEVELDNQIVTATQSNICDEDSATETCYTYDRNKCYTAVVPLVYGGETKMVETALTPDACYPD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides
别名 IGCJ, Immunoglobulin J chain, JCH, JCHAIN, Joining chain of multimeric IgA and IgM
计算分子量 158 aa, 18 kDa
观测分子量25 kDa
GenBank蛋白编号BC038982
基因名称 IGJ
Gene ID (NCBI) 3512
RRIDAB_3745221
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01591
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IGJ, the immunoglobulin J chain, serves to link two monomer units of either IgM or IgA.

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