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验证数据展示

  • IHC staining of human tonsillitis using Biotin-67538

    IHC staining of human tonsillitis using Biotin-67538

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-67538 (human IgD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with human IgD antibody included

Biotin-conjugated IgD Monoclonal antibody

human IgD Monoclonal Antibody for IHC
Cat No. Biotin-67538

产品说明书

CloneNo. 1D1B12

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

IHC

别名

FLJ00382, FLJ46727, IGHD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy human IgD Ready-To-Use IHC Kit. human IgD primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-67538 targets human IgD in IHC applications and shows reactivity with Human samples.

经测试应用 IHC Application Description
经测试反应性 Human
免疫原

CatNo: Ag9614

Product name: Recombinant human IGHD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 202-548 aa of BC021276

Sequence: RRDSYYMTSSQLSTPLQQWRQGEYKCVVQHTASKSKKEIFRWPESPKAQASSVPTAQPQAEGSLAKATTAPATTRNTGRGGEEKKKEKEKEEQEERETKTPECPSHTQPLGVYLLTPAVQDLWLRDKATFTCFVVGSDLKDAHLTWEVAGKVPTGGVEEGLLERHSNGSQSQHSRLTLPRSLWNAGTSVTCTLNHPSLPPQRLMALREPAAQAPVKLSLNLLASSDPPEAASWLLCEVSGFSPPNILLMWLEDQREVNTSGFAPARPPPQPGSTTFWAWSVLRVPAPPSPQPATYTCVVSHEDSRTLLNASRSLEVSYLAMTPLIPQSKDENSDDYTTFDDVGSLWT

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 immunoglobulin heavy constant delta
别名 FLJ00382, FLJ46727, IGHD
计算分子量 573 aa, 63 kDa
观测分子量 55-70 kDa
GenBank蛋白编号BC021276
基因名称 IGHD
Gene ID (NCBI) 3495
RRIDAB_2918950
偶联类型 Biotin
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells. The function of IgD is to signal the B cells to be activated. The relative molecular mass and half-life of secreted IgD is 185 kDa and 2.8 days, respectively. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta (δ) class, and two Ig light chains. This antibody detects the heavy chain (55-70 kDa) of IgD and secreted IgD (185 kDa) .

实验方案

Product Specific Protocols
IHC protocol for Biotin human IgD antibody Biotin-67538Download protocol
Standard Protocols
Click here to view our Standard Protocols