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验证数据展示

  • WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83200-3-RR (IGFBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83200-3-RR (IGFBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83200-3-RR (same clone as 83200-3-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83200-3-RR (same clone as 83200-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83200-3-RR (IGFBP4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83200-3-RR (same clone as 83200-3-PBS)

    FC experiment of A549 using 83200-3-RR (same clone as 83200-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.4 ug IGFBP4 Recombinant antibody (83200-3-RR, Clone:230543F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83200-3-RR

    Affinity and Kinetic Characterization of 83200-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83200-3-RR against Human IGFBP4 were performed. The affinity constant is 0.776 nM.

IGFBP4 Recombinant monoclonal antibody, PBS Only

IGFBP4 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 83200-3-PBS

产品说明书

CloneNo. 230543F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

230543F8, BP 4, BP4, Deb2, HT29 IGFBP

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83200-3-PBS targets IGFBP4 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0825

Product name: Recombinant Human IGFBP-4 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 22-258 aa of NM_001552.3

Sequence: DEAIHCPPCSEEKLARCRPPVGCEELVREPGCGCCATCALGLGMPCGVYTPRCGSGLRCYPPRGVEKPLHTLMHGQGVCMELAEIEAIQESLQPSDKDEGDHPNNSFSPCSAHDRRCLQKHFAKIRDRSTSGGKMKVNGAPREDARPVPQGSCQSELHRALERLAASQSRTHEDLYIIPIPNCDRNGNFHPKQCHPALDGQRGKCWCVDRKTGVKLPGGLEPKGELDCHQLADSFRE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 insulin-like growth factor binding protein 4
别名 230543F8, BP 4, BP4, Deb2, HT29 IGFBP
计算分子量28 kDa
观测分子量 28-40 kDa
GenBank蛋白编号NM_001552.3
基因名称 IGFBP4
Gene ID (NCBI) 3487
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP22692-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP4 is the smallest member of the insulin-like growth factor binding protein family (IGFBP). It is a hepatic protein that plays a role in modulating the activity and bioavailability of IGF-I. The expression of IGFBP4 was found to increase under conditions of hypoxia. IGFBP4 is highly expressed in adipocytes and osteoblasts and is inhibitory of IGFs in vitro. (PMID: 28938423, PMID: 34646401).

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