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验证数据展示

  • WB analysis using 84385-3-RR

    WB analysis using 84385-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84385-3-RR (IGF1R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 84385-3-RR

    IF Staining of MCF-7 using 84385-3-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IGF1R antibody (84385-3-RR, Clone: 241678B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84385-3-RR

    Affinity and Kinetic Characterization of 84385-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84385-3-RR against Human IGF1R were performed. The affinity constant is 0.239 nM.

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IGF1R/CD221 Recombinant monoclonal antibody

IGF1R/CD221 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84385-3-RR

产品说明书

CloneNo. 241678B8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

IGF 1 receptor, IGF1R, IGF-I receptor, IGFIR, IGFR

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, A549 cells, L02 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy IGF1R Ready-To-Use IHC Kit. IGF1R primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84385-3-RR targets IGF1R/CD221 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IGF I receptor
别名 IGF 1 receptor, IGF1R, IGF-I receptor, IGFIR, IGFR
计算分子量 1367 aa, 155 kDa
观测分子量130 kDa
GenBank蛋白编号BC113612
基因名称 IGF1R
Gene ID (NCBI) 3480
RRIDAB_3671921
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP08069
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is translated as ~220 kDa pro-receptors (PMID: 33969359). IGF1R is a heterotetrameric receptor, composed of two alpha subunits (~130 kDa) and two beta subunits (~100-120 kDa) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.

实验方案

Product Specific Protocols
WB protocol for IGF1R/CD221 antibody 84385-3-RRDownload protocol
IF protocol for IGF1R/CD221 antibody 84385-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols