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IGF1R Polyclonal antibody

IGF1R Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 21707-1-AP

Print datasheet

Synonyms

CD221, IGF I receptor, IGF1R, IGF1R alpha chain, IGFIR, IGFR, JTK13

验证数据展示

  • WB analysis using 21707-1-AP

    WB analysis using 21707-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21707-1-AP (IGF1R antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy IGF1R Ready-To-Use IHC Kit. IGF1R primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21707-1-AP targets IGF1R in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen IGF1R fusion protein Ag16325 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name IGF I receptor
Synonyms CD221, IGF I receptor, IGF1R, IGF1R alpha chain, IGFIR, IGFR, JTK13
Calculated Molecular Weight 1367 aa, 155 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC113612
Gene Symbol IGF1R
Gene ID (NCBI) 3480
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08069
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is a heterotetrameric receptor, composed of two alpha subunits (130 kDa each) and two beta subunits (~98 kDa each) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.

实验方案

Product Specific Protocols
WB protocol for IGF1R antibody 21707-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols