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验证数据展示

  • FC experiment of A549 using 98889-3-RR (same clone as 98889-3-PBS)

    FC experiment of A549 using 98889-3-RR (same clone as 98889-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Anti-Human IGF1 Rabbit RecAb (98889-3-RR, Clone: 252943B2) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98889-3-PBS in a different storage buffer formulation.

  • FC experiment of Transfected HEK-293T using 98889-3-RR (same clone as 98889-3-PBS)

    FC experiment of Transfected HEK-293T using 98889-3-RR (same clone as 98889-3-PBS)

    1x10^6 HEK-293T cells and IGF1 Transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human IGF1 Rabbit RecAb (98889-3-RR, Clone: 252943B2) and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98889-3-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 98889-3-RR (same clone as 98889-3-PBS)

    IHC staining of human liver using 98889-3-RR (same clone as 98889-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 98889-3-RR (IGF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98889-3-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 98889-3-RR (same clone as 98889-3-PBS)

    IHC staining of human liver using 98889-3-RR (same clone as 98889-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 98889-3-RR (IGF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98889-3-PBS in a different storage buffer formulation.

  • FC experiment of Transfected HEK-293T using 98889-3-RR (same clone as 98889-3-PBS)

    FC experiment of Transfected HEK-293T using 98889-3-RR (same clone as 98889-3-PBS)

    1x10^6 HEK-293T cells and IGF1 Transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human IGF1 Rabbit RecAb (98889-3-RR, Clone: 252943B2) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98889-3-PBS in a different storage buffer formulation.

Anti-Human IGF1 Rabbit Recombinant Antibody, PBS Only

IGF1 Uni-rAb® Recombinant Antibody for IHC, FC (Intra)
Cat No. 98889-3-PBS

产品说明书

CloneNo. 252943B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC (Intra)

别名

IGF-1, IGF I, IGFI, IGF-I, Insulin like growth factor I

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98889-3-PBS targets IGF1 in IHC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IHC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Eg1806

Product name: recombinant human IGF1 protein

Source: mammalian cells-derived, pHZ-KIsec-N-mFc

Tag: N-mFc

Domain: 49-118 aa of NM_001111285.2

Sequence: EPRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVTCVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEFKCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIYVEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHTTKSFSRTPGK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 insulin-like growth factor 1 (somatomedin C)
别名 IGF-1, IGF I, IGFI, IGF-I, Insulin like growth factor I
计算分子量 22 kDa
GenBank蛋白编号NM_001111285.2
基因名称 IGF1
Gene ID (NCBI) 3479
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05019-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Insulin like growth factor-1, also known as Somatomedin-C is a growth factor which is structurally related to insulin and is an important regulator of growth and differentiation in various tissues and cell systems. Human IGF-1 is synthesized as two precursor isoforms with N- and alternate C-terminal propeptide. The two precursor isoforms are differentially expressed by various tissues. The proteolytic cleavage of the N- and C-terminal regions results in the mature IGF-1 protein which is identical between isoforms. IGF-1 binds to IGF-1 receptor and induces receptor autophosphorylation. This further phosphorylates Insulin receptor substrate -1 (IRS-1) and activates various downstream signaling pathways including the PI3-AKT, MAPK etc. (PMID: 17354613, 17113337, 29535161)

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