• Featured Product
  • KD/KO验证

IFN-gamma Polyclonal antibody

IFN-gamma Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15365-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IHC, ELISA and More (1)

IFNG, IFG, IFN gamma, IFN γ, IFNγ

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inNK-92 cells, Recombinant protein
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15365-1-AP targets IFN-gamma in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, ELISA
经测试反应性 human
文献引用反应性human, rat, pig
免疫原 IFN-gamma fusion protein Ag8321 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IFN gamma
别名 IFNG, IFG, IFN gamma, IFN γ, IFNγ
计算分子量 166 aa, 19 kDa
观测分子量22 kDa
GenBank蛋白编号BC070256
基因名称 IFNG
Gene ID (NCBI) 3458
ENSEMBL Gene IDENSG00000111537
RRIDAB_2123037
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01579
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFN gamma (IFNG) is a soluble cytokine that is the only member of the type II class of IFN. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

实验方案

Product Specific Protocols
WB protocol for IFN-gamma antibody 15365-1-APDownload protocol
IHC protocol for IFN-gamma antibody 15365-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Immunol

Targeting regulator of G protein signaling 1 in tumor-specific T cells enhances their trafficking to breast cancer.

Authors - Di Huang
humanIF

Adv Sci (Weinh)

Targeting Deltex E3 Ubiquitin Ligase 2 Inhibits Tumor-associated Neutrophils and Sensitizes Hepatocellular Carcinoma Cells to Immunotherapy

Authors - Xiaoling Wu
IF

Acta Pharm Sin B

Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy

Authors - Hao Ren
humanIF

Gastroenterology

Interferon-γ-stimulated genes, but not USP18, are expressed in livers of patients with acute hepatitis C.

Authors - Michael T Dill
WB,IHC

eBioMedicine

Histone deacetylases inhibitor chidamide synergizes with humanized PD1 antibody to enhance T-cell chemokine expression and augment Ifn-γ response in NK-T cell lymphoma

Authors - Tingyu Wen
WB

J Control Release

Efficient gene delivery by multifunctional star poly (β-amino ester)s into difficult-to-transfect macrophages for M1 polarization

Authors - Tao Bo
Loading...