验证数据展示
经过测试的应用
| Positive WB detected in | NK-92 cells, Recombinant protein |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15365-1-AP targets IFN-gamma in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ELISA |
| 经测试反应性 | human |
| 文献引用反应性 | human, rat, pig |
| 免疫原 |
CatNo: Ag8321 Product name: Recombinant human IFNG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-166 aa of BC070256 Sequence: YCQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRGRRASQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | IFN gamma |
| 别名 | IFNG, IFG, IFN gamma, IFN γ, IFNγ |
| 计算分子量 | 166 aa, 19 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC070256 |
| 基因名称 | IFNG |
| Gene ID (NCBI) | 3458 |
| ENSEMBL Gene ID | ENSG00000111537 |
| RRID | AB_2123037 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P01579 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFN gamma (IFNG) is a soluble cytokine that is the only member of the type II class of IFN. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IFN-gamma antibody 15365-1-AP | Download protocol |
| IHC protocol for IFN-gamma antibody 15365-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Immunol Targeting regulator of G protein signaling 1 in tumor-specific T cells enhances their trafficking to breast cancer. | ||
Adv Sci (Weinh) Targeting Deltex E3 Ubiquitin Ligase 2 Inhibits Tumor-associated Neutrophils and Sensitizes Hepatocellular Carcinoma Cells to Immunotherapy | ||
Acta Pharm Sin B Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy | ||
Gastroenterology Interferon-γ-stimulated genes, but not USP18, are expressed in livers of patients with acute hepatitis C. | ||
eBioMedicine Histone deacetylases inhibitor chidamide synergizes with humanized PD1 antibody to enhance T-cell chemokine expression and augment Ifn-γ response in NK-T cell lymphoma | ||
J Control Release Efficient gene delivery by multifunctional star poly (β-amino ester)s into difficult-to-transfect macrophages for M1 polarization |
