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验证数据展示

  • WB analysis using 15365-1-AP (same clone as 15365-1-PBS)

    WB analysis using 15365-1-AP (same clone as 15365-1-PBS)

    PMA, Ionomycin and Brefeldin A treated NK-92 cells were subjected to SDS PAGE followed by western blot with 15365-1-AP (IFN Gamma antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15365-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 15365-1-AP (same clone as 15365-1-PBS)

    IHC staining of human tonsillitis using 15365-1-AP (same clone as 15365-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15365-1-AP (IFN Gamma antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15365-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

IFN-gamma Polyclonal antibody, PBS Only

IFN-gamma Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15365-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

IFNG, IFG, IFN gamma, IFN γ, IFNγ

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

15365-1-PBS targets IFN-gamma in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8321

Product name: Recombinant human IFNG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 22-166 aa of BC070256

Sequence: YCQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRGRRASQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IFN gamma
别名 IFNG, IFG, IFN gamma, IFN γ, IFNγ
计算分子量 166 aa, 19 kDa
观测分子量22 kDa
GenBank蛋白编号BC070256
基因名称 IFNG
Gene ID (NCBI) 3458
ENSEMBL Gene IDENSG00000111537
RRIDAB_2123037
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01579
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IFN gamma (IFNG) is a soluble cytokine that is the only member of the type II class of IFN. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

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