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验证数据展示

  • FC experiment of human blood using 98474-5-RR

    FC experiment of human blood using 98474-5-RR

    1x10^6 human blood were surface stained with 0.25 ug Anti-Human IFNAR2 Rabbit RecAb (98474-5-RR, Clone: 250313D4) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human blood using 98474-5-RR

    FC experiment of human blood using 98474-5-RR

    1x10^6 human blood cells were surface stained with 0.25 ug Anti-Human IFNAR2 Rabbit RecAb (98474-5-RR, Clone: 250313D4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity of 98474-5-RR

    Affinity of 98474-5-RR

    Biolayer interferometry (BLl) kinetic assays of 98474-5-RR against Human IFNAR2 were performed. The affinity constant is below 1 pM.

Anti-Human IFNAR2 Rabbit Recombinant Antibody

IFNAR2 Uni-rAb® Recombinant Antibody for FC
Cat No. 98474-5-RR

产品说明书

CloneNo. 250313D4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

IFN alpha REC, IFN alpha/beta receptor 2, IFN R, IFN R 2, IFNABR

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman blood cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98474-5-RR targets IFNAR2 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg2928

Product name: Recombinant Human IFNAR2 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 27-243 aa of NM_207585.2

Sequence: ISYDSPDYTDESCTFKISLRNFRSILSWELKNHSIVPTHYTLLYTIMSKPEDLKVVKNCANTTRSFCDLTDEWRSTHEAYVTVLEGFSGNTTLFSCSHNFWLAIDMSFEPPEFEIVGFTNHINVMVKFPSIVEEELQFDLSLVIEEQSEGIVKKHKPEIKGNMSGNFTYIIDKLIPNTNYCVSVYLEHSDEQAVIKSPLKCTLLPPGQESESAESAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interferon (alpha, beta and omega) receptor 2
别名 IFN alpha REC, IFN alpha/beta receptor 2, IFN R, IFN R 2, IFNABR
计算分子量58 kDa
GenBank蛋白编号NM_207585.2
基因名称 IFNAR2
Gene ID (NCBI) 3455
RRIDAB_3746104
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP48551-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Interferon alpha/beta receptor 2 (IFNAR2) is a type I membrane protein that associates with IFNAR1 to form the receptor for type I interferons. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2, and then increase transcription of the IFN-induced genes whose products exert antiviral, immunomodulatory, and antiproliferative effects.

实验方案

Product Specific Protocols
FC protocol for IFNAR2 antibody 98474-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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