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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 81135-3-RR (same clone as 81135-3-PBS)

    WB analysis using 81135-3-RR (same clone as 81135-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81135-3-RR (IFITM3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81135-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 81135-3-RR (same clone as 81135-3-PBS)

    IF Staining of HepG2 using 81135-3-RR (same clone as 81135-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IFITM3 antibody (81135-3-RR, Clone: 242056C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81135-3-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 81135-3-RR (same clone as 81135-3-PBS)

    FC experiment of MCF-7 using 81135-3-RR (same clone as 81135-3-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug IFITM3 Recombinant antibody (81135-3-RR, Clone:242056C9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81135-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81135-3-PBS

    Affinity and Kinetic Characterization of 81135-3-PBS

    Biolayer interferometry (BLl) kinetic assays of 81135-3-RR against Human IFITM3 were performed. The affinity constant is 0.853 nM.

  • Affinity and Kinetic Characterization of 81135-3-RR

    Affinity and Kinetic Characterization of 81135-3-RR

    Biolayer interferometry (BLl) kinetic assays of 81135-3-RR against Human IFITM3 were performed. The affinity constant is 0.911 nM.

IFITM3 Recombinant antibody, PBS Only

IFITM3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 81135-3-PBS

产品说明书

CloneNo. 242056C9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

Interferon-inducible protein 1-8U, Interferon-induced transmembrane protein 3, IFITM2/3, DSPA2b, 242056C9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IFITM2/3 Ready-To-Use IHC Kit. IFITM2/3 primary antibody included.

产品信息

81135-3-PBS targets IFITM3 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 IFITM3 fusion protein Ag2285 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interferon induced transmembrane protein 3 (1-8U)
别名 Interferon-inducible protein 1-8U, Interferon-induced transmembrane protein 3, IFITM2/3, DSPA2b, 242056C9
计算分子量 133 aa, 15 kDa
观测分子量15-20 kDa
GenBank蛋白编号BC006794
基因名称 IFITM3
Gene ID (NCBI) 10410
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ01628
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression.