验证数据展示
经过测试的应用
Positive WB detected in | IFN alpha treated HeLa cells, SGC-7901 cells |
Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83423-1-RR targets IFIT1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
经测试反应性 | human |
免疫原 | IFIT1 fusion protein Ag19691 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | interferon-induced protein with tetratricopeptide repeats 1 |
别名 | IFIT 1, IFI-56K, IFI56, IFI 56K, G10P1 |
计算分子量 | 478 aa, 55 kDa |
观测分子量 | 55-65 kDa |
GenBank蛋白编号 | BC007091 |
基因名称 | IFIT1 |
Gene ID (NCBI) | 3434 |
RRID | AB_3671072 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P09914 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFIT1, also known as glucocorticoid-attenuated response gene 16 protein (GARG-16), is a 463 amino acid protein belonging to the IFIT family. IFIT genes comprise a large family with three (IFIT1, IFIT2, and IFIT3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT1 specifically bound virus PPP-RNA forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent virus replication.
实验方案
Product Specific Protocols | |
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WB protocol for IFIT1 antibody 83423-1-RR | Download protocol |
IHC protocol for IFIT1 antibody 83423-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |