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验证数据展示

  • WB analysis of HeLa using 83423-1-RR (same clone as 83423-1-PBS)

    WB analysis of HeLa using 83423-1-RR (same clone as 83423-1-PBS)

    IFN alpha treated HeLa cells were subjected to SDS PAGE followed by western blot with 83423-1-RR (IFIT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83423-1-RR (same clone as 83423-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83423-1-RR (same clone as 83423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83423-1-RR (IFIT1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83423-1-RR (same clone as 83423-1-PBS)

    FC experiment of HeLa using 83423-1-RR (same clone as 83423-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ifit1 Recombinant Antibody (83423-1-RR, Clone:240132G11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83423-1-RR

    Affinity and Kinetic Characterization of 83423-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83423-1-RR against Human IFIT1 were performed. The affinity constant is 0.397 nM.

IFIT1 Recombinant antibody, PBS Only

IFIT1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83423-1-PBS

产品说明书

CloneNo. 240132G11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

IFIT 1, IFI-56K, IFI56, IFI 56K, G10P1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83423-1-PBS targets IFIT1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 IFIT1 fusion protein Ag19691 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interferon-induced protein with tetratricopeptide repeats 1
别名 IFIT 1, IFI-56K, IFI56, IFI 56K, G10P1
计算分子量 478 aa, 55 kDa
观测分子量 55-65 kDa
GenBank蛋白编号BC007091
基因名称 IFIT1
Gene ID (NCBI) 3434
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP09914
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IFIT1, also known as glucocorticoid-attenuated response gene 16 protein (GARG-16), is a 463 amino acid protein belonging to the IFIT family. IFIT genes comprise a large family with three (IFIT1, IFIT2, and IFIT3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT1 specifically bound virus PPP-RNA forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent virus replication.