IFIH1-Specific Polyclonal antibody
IFIH1-Specific Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy IFIH1 Ready-To-Use IHC Kit. IFIH1 primary antibody included.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16879-1-AP targets IFIH1-Specific in WB, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, ELISA |
Tested Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | interferon induced with helicase C domain 1 |
Synonyms | CADM-140 autoantigen, Helicard, Helicase with 2 CARD domains, Hlcd, IDDM19, IFIH1, IFIH1-Specific, MDA 5, MDA5, RH116, RLR-2 |
Calculated Molecular Weight | 117 kDa |
Observed Molecular Weight | 70 kDa, 90 kDa |
GenBank Accession Number | NM_022168 |
Gene Symbol | IFIH1 |
Gene ID (NCBI) | 64135 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9BYX4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501). This antibody is specific to IFIH1.
实验方案
Product Specific Protocols | |
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WB protocol for IFIH1-Specific antibody 16879-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |