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- KD/KO Validated
IFIH1/MDA5 Polyclonal antibody
IFIH1/MDA5 Polyclonal Antibody for IF-P, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
IF-P, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, Raji cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse skeletal muscle tissue |
Planning an IHC experiment? We recommend our IHCeasy IFIH1 Ready-To-Use IHC Kit. IFIH1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21775-1-AP targets IFIH1/MDA5 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF-P, IHC, IP, WB, ELISA |
| Cited Applications | IF, IHC, IP, RIP, WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, monkey, pig |
| Immunogen | IFIH1/MDA5 fusion protein Ag16467 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | interferon induced with helicase C domain 1 |
| Synonyms | Helicard, Helicase with 2 CARD domains, Hlcd, IDDM19, IFIH1, IFIH1/MDA5, MDA 5, MDA5, RH116, RLR-2 |
| Calculated Molecular Weight | 1025 aa, 117 kDa |
| Observed Molecular Weight | 117-125 kDa |
| GenBank Accession Number | BC111750 |
| Gene Symbol | IFIH1 |
| Gene ID (NCBI) | 64135 |
| RRID | AB_10734593 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYX4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IFIH1/MDA5 antibody 21775-1-AP | Download protocol |
| IHC protocol for IFIH1/MDA5 antibody 21775-1-AP | Download protocol |
| IF protocol for IFIH1/MDA5 antibody 21775-1-AP | Download protocol |
| IP protocol for IFIH1/MDA5 antibody 21775-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion | ||
Cell Stem Cell ADAR1-Dependent RNA Editing Promotes MET and iPSC Reprogramming by Alleviating ER Stress. | ||
Nat Commun MLL5 suppresses antiviral innate immune response by facilitating STUB1-mediated RIG-I degradation. | ||
Nat Commun HIV-1 intron-containing RNA expression induces innate immune activation and T cell dysfunction. | ||
EMBO J CircPVT1 promotes ER-positive breast tumorigenesis and drug resistance by targeting ESR1 and MAVS
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