验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Daudi cells, HL-60 cells, Raji cells, rat spleen tissue |
Planning an IHC experiment? We recommend our IHCeasy IFI30 Ready-To-Use IHC Kit. IFI30 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85982-1-RR targets IFI30 in WB, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 | IFI30 fusion protein Ag2178 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interferon, gamma-inducible protein 30 |
| 别名 | EC:1.8.-.-, Gamma-interferon-inducible lysosomal thiol reductase, Gamma-interferon-inducible protein IP-30, GILT, IFI 30 |
| 计算分子量 | 261 aa, 29 kDa |
| 观测分子量 | 30-35 kDa |
| GenBank蛋白编号 | BC021136 |
| 基因名称 | IFI30 |
| Gene ID (NCBI) | 10437 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P13284 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFI30(interferon, gamma-inducible protein 30) is also named as IP30, GILT, IFI-30, MGC32056, IFI30 and belongs to the GILT family. It can facilitate the processing and presentation to antigen-specific T cells of protein antigens containing disulfide bonds. IFI30 is inducibly up-regulated by IFN-g, as are MHC class II molecules, the associated invariant chain, and HLA-DM, which catalyses peptide loading in MIICs and the 35 kDa species is the IFI30 proform, and the 30-kDa species is the mature form(PMID:10639150). It can exsit as a dimer(PMID:17142755).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IFI30 antibody 85982-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |