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  • Featured Product
  • KD/KO Validated

IFI30 Polyclonal antibody

IFI30 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11597-1-AP

Print datasheet

Synonyms

GILT, IFI 30, IFI30, IP30

验证数据展示

  • IP experiment of COLO 320 using 11597-1-AP

    IP experiment of COLO 320 using 11597-1-AP

    IP result of anti-IFI30 (IP:11597-1-AP, 4ug; Detection:11597-1-AP 1:3000) with COLO 320 cells lysate 2400 ug.

  • IF Staining of HeLa using 11597-1-AP

    IF Staining of HeLa using 11597-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 11597-1-AP (IFI30 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of COLO 320 using 11597-1-AP

    WB analysis of COLO 320 using 11597-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 11597-1-AP (IFI30 Antibody) at dilution of 1:6000 incubated at 4 degree celsius over night.

  • IHC staining of human liver cancer using 11597-1-AP

    IHC staining of human liver cancer using 11597-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11597-1-AP (IFI30 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFI30 antibody included
  • IHC staining of human liver cancer using 11597-1-AP

    IHC staining of human liver cancer using 11597-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11597-1-AP (IFI30 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFI30 antibody included

经过测试的应用

Positive WB detected inCOLO 320 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IFI30 Ready-To-Use IHC Kit. IFI30 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11597-1-AP targets IFI30 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen IFI30 fusion protein Ag2178 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon, gamma-inducible protein 30
Synonyms GILT, IFI 30, IFI30, IP30
Calculated Molecular Weight 261 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC021136
Gene Symbol IFI30
Gene ID (NCBI) 10437
RRIDAB_2121789
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13284
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFI30(interferon, gamma-inducible protein 30) is also named as IP30, GILT, IFI-30, MGC32056, IFI30 and belongs to the GILT family. It can facilitate the processing and presentation to antigen-specific T cells of protein antigens containing disulfide bonds. IFI30 is inducibly up-regulated by IFN-g, as are MHC class II molecules, the associated invariant chain, and HLA-DM, which catalyses peptide loading in MIICs and the 35 kDa species is the IFI30 proform, and the 30-kDa species is the mature form(PMID:10639150). It can exsit as a dimer(PMID:17142755).

实验方案

Product Specific Protocols
WB protocol for IFI30 antibody 11597-1-APDownload protocol
IHC protocol for IFI30 antibody 11597-1-APDownload protocol
IF protocol for IFI30 antibody 11597-1-APDownload protocol
IP protocol for IFI30 antibody 11597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Int J Med Sci

IFI30 expression predicts patient prognosis in breast cancer and dictates breast cancer cells proliferation via regulating autophagy.

Authors - Yan Fan
  • KD Validated
View Article
humanWB

Phytomedicine

Nobiletin inhibits de novo FA synthesis to alleviate gastric cancer progression by regulating endoplasmic reticulum stress

Authors - Menglin Chen
View Article