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验证数据展示

  • WB analysis using 82117-1-RR

    WB analysis using 82117-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82117-1-RR (IDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 82117-1-RR

    WB analysis using 82117-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82117-1-RR (IDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 82117-1-RR

    WB analysis of HEK-293 using 82117-1-RR

    WB result of IDH2 antibody (82117-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDH2 transfected HEK-293 cells.

  • IP experiment of mouse brain using 82117-1-RR

    IP experiment of mouse brain using 82117-1-RR

    IP result of anti-IDH2 (IP:82117-1-RR, 4ug; Detection:82117-1-RR 1:1000) with mouse brain tissue lysate 1280 ug.

  • IHC staining of human colon cancer using 82117-1-RR

    IHC staining of human colon cancer using 82117-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82117-1-RR (IDH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with IDH2 antibody included
  • Featured Product
  • KD/KO验证
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IDH2 Recombinant monoclonal antibody

IDH2 Uni-rAb® Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 82117-1-RR

产品说明书

CloneNo. 1B1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

IDHM, IDH 2, ICD-M, ICD M, EC:1.1.1.42

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, mouse heart tissue, Jurkat cells, rat heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IDH2 Ready-To-Use IHC Kit. IDH2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82117-1-RR targets IDH2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8779

Product name: Recombinant human IDH2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 104-452 aa of BC009244

Sequence: TQKYSVAVKCATITPDEARVEEFKLKKMWKSPNGTIRNILGGTVFREPIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVADRAGTFKMVFTPKDGSGVKEWEVYNFPAGGVGMGMYNTDESISGFAHSCFQYAIQKKWPLYMSTKNTILKAYDGRFKDIFQEIFDKHYKTDFDKNKIWYEHRLIDDMVAQVLKSSGGFVWACKNYDGDVQSDILAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQMLEKVCVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALGRQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 isocitrate dehydrogenase 2 (NADP+), mitochondrial
别名 IDHM, IDH 2, ICD-M, ICD M, EC:1.1.1.42
计算分子量 452 aa, 51 kDa
观测分子量43 kDa
GenBank蛋白编号BC009244
基因名称 IDH2
Gene ID (NCBI) 3418
RRIDAB_3086464
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP48735
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.

实验方案

Product Specific Protocols
WB protocol for IDH2 antibody 82117-1-RRDownload protocol
IHC protocol for IDH2 antibody 82117-1-RRDownload protocol
IP protocol for IDH2 antibody 82117-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols