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验证数据展示

  • WB analysis using 66918-1-Ig (same clone as 66918-1-PBS)

    WB analysis using 66918-1-Ig (same clone as 66918-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66918-1-Ig (IDH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66918-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66918-1-Ig (same clone as 66918-1-PBS)

    WB analysis of HEK-293 using 66918-1-Ig (same clone as 66918-1-PBS)

    WB result of IDH2 antibody (66918-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDH2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66918-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66918-1-Ig (same clone as 66918-1-PBS)

    FC experiment of HepG2 using 66918-1-Ig (same clone as 66918-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IDH2 Monoclonal antibody (66918-1-Ig, Clone:1F10A10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. This data was developed using the same antibody clone with 66918-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

IDH2 Monoclonal antibody, PBS Only

IDH2 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 66918-1-PBS

产品说明书

CloneNo. 1F10A10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

IDHM, IDH 2, ICD-M, ICD M, EC:1.1.1.42

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IDH2 Ready-To-Use IHC Kit. IDH2 primary antibody included.

产品信息

66918-1-PBS targets IDH2 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8950

Product name: Recombinant human IDH2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 104-452 aa of BC009244

Sequence: TQKYSVAVKCATITPDEARVEEFKLKKMWKSPNGTIRNILGGTVFREPIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVADRAGTFKMVFTPKDGSGVKEWEVYNFPAGGVGMGMYNTDESISGFAHSCFQYAIQKKWPLYMSTKNTILKAYDGRFKDIFQEIFDKHYKTDFDKNKIWYEHRLIDDMVAQVLKSSGGFVWACKNYDGDVQSDILAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQMLEKVCVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALGRQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 isocitrate dehydrogenase 2 (NADP+), mitochondrial
别名 IDHM, IDH 2, ICD-M, ICD M, EC:1.1.1.42
计算分子量 452 aa, 51 kDa
观测分子量 41-47 kDa
GenBank蛋白编号BC009244
基因名称 IDH2
Gene ID (NCBI) 3418
RRIDAB_2882245
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP48735
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.