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验证数据展示

  • WB analysis using 81176-1-RR (same clone as 81176-1-PBS)

    WB analysis using 81176-1-RR (same clone as 81176-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81176-1-RR (IDH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81176-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 81176-1-RR (same clone as 81176-1-PBS)

    FC experiment of HepG2 using 81176-1-RR (same clone as 81176-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IDH1 Recombinant antibody (81176-1-RR, Clone:6L18) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 81176-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 81176-1-RR (same clone as 81176-1-PBS)

    IHC staining of human gliomas using 81176-1-RR (same clone as 81176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 81176-1-RR (IDH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81176-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDH1 antibody included
  • IHC staining of human gliomas using 81176-1-RR (same clone as 81176-1-PBS)

    IHC staining of human gliomas using 81176-1-RR (same clone as 81176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 81176-1-RR (IDH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81176-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDH1 antibody included
  • Affinity and Kinetic Characterization of 81176-1-RR

    Affinity and Kinetic Characterization of 81176-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81176-1-RR against Human IDH1 were performed. The affinity constant is 17.5 pM.

IDH1 Recombinant monoclonal antibody, PBS Only

IDH1 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 81176-1-PBS

产品说明书

CloneNo. 6L18

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

6L18, Cytosolic NADP-isocitrate dehydrogenase, EC:1.1.1.42, IDCD, IDH

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IDH1 Ready-To-Use IHC Kit. IDH1 primary antibody included.

产品信息

81176-1-PBS targets IDH1 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19749

Product name: Recombinant human IDH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 341-414 aa of BC012846

Sequence: AHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 isocitrate dehydrogenase 1 (NADP+), soluble
别名 6L18, Cytosolic NADP-isocitrate dehydrogenase, EC:1.1.1.42, IDCD, IDH
计算分子量 414 aa, 47 kDa
观测分子量 46 kDa
GenBank蛋白编号BC012846
基因名称 IDH1
Gene ID (NCBI) 3417
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75874
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171). IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).

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