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验证数据展示

  • WB analysis of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    WB analysis of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66197-1-Ig (same clone as 66197-1-PBS)

    IHC staining of human liver cancer using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66197-1-Ig (IDH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDH1 antibody included
  • IHC staining of human liver cancer using 66197-1-Ig (same clone as 66197-1-PBS)

    IHC staining of human liver cancer using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66197-1-Ig (IDH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDH1 antibody included
  • IF Staining of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    IF Staining of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66197-1-Ig (IDH1 antibody) at dilution of 1:400 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    IF Staining of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66197-1-Ig(IDH1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    FC experiment of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IDH1 Monoclonal antibody (66197-1-Ig, Clone:2A6A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51184-1

    Cytometric bead array standard curve of MP51184-1

    Cytometric bead array standard curve of MP51184-1, IDH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66197-2-PBS. Detection antibody: 66197-1-PBS. Standard:Ag19293. Range: 3.125-200 ng/mL.

IDH1 Monoclonal antibody, PBS Only (Detector)

IDH1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 66197-1-PBS

产品说明书

CloneNo. 2A6A2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

IDH 1, IDH, IDCD, EC:1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IDH1 Ready-To-Use IHC Kit. IDH1 primary antibody included.

产品信息

66197-1-PBS targets IDH1 as part of a matched antibody pair:

MP51184-1: 66197-2-PBS capture and 66197-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 IDH1 fusion protein Ag19293 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 isocitrate dehydrogenase 1 (NADP+), soluble
别名 IDH 1, IDH, IDCD, EC:1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase
计算分子量 414 aa, 47 kDa
观测分子量 46 kDa
GenBank蛋白编号BC012846
基因名称 IDH1
Gene ID (NCBI) 3417
RRIDAB_2881590
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75874
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).