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验证数据展示

  • WB analysis using 23309-1-AP (same clone as 23309-1-PBS)

    WB analysis using 23309-1-AP (same clone as 23309-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23309-1-AP (IDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23309-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 23309-1-AP (same clone as 23309-1-PBS)

    IHC staining of mouse kidney using 23309-1-AP (same clone as 23309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23309-1-AP (IDH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23309-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDH1 antibody included

IDH1 Polyclonal antibody, PBS Only

IDH1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 23309-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IDH1 Ready-To-Use IHC Kit. IDH1 primary antibody included.

产品信息

23309-1-PBS targets IDH1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19749

Product name: Recombinant human IDH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 341-414 aa of BC012846

Sequence: AHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 isocitrate dehydrogenase 1 (NADP+), soluble
别名
计算分子量 414 aa, 47 kDa
观测分子量 46 kDa
GenBank蛋白编号BC012846
基因名称 IDH1
Gene ID (NCBI) 3417
RRIDAB_11232028
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75874
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. Its subunit structure is homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ). This antibody is specific to IDH1.