验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A375 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells, A431 cells, HEK-293 cells, MCF-7 cells, NCCIT cells, HT-1080 cells, LNCaP cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
67106-1-Ig targets IDE in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag17161 Product name: Recombinant human IDE protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 676-1019 aa of BC096336 Sequence: EQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Abeta degrading protease |
| 别名 | Insulin-degrading enzyme, Insulinase, Insulin protease, EC:3.4.24.56, Abeta-degrading protease |
| 计算分子量 | 1019 aa, 118 kDa |
| 观测分子量 | 100-120 kDa |
| GenBank蛋白编号 | BC096336 |
| 基因名称 | IDE |
| Gene ID (NCBI) | 3416 |
| RRID | AB_2882410 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P14735 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IDE(INS-degrading enzyme) belongs to the peptidase M16 family. It is also known as insulysin and is a 110-kD neutral metallopeptidase that can degrade a number of peptides, including INS and beta-amyloid(PMID:9830016). The reduction of IDE expression may be a risk factor for AD, and that IDE may interact with APOE status to affect beta-amyloid metabolism(PMID:12507914). It can exsit as a homodimer and form higher oligomers(PMID:17613531).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IDE antibody 67106-1-Ig | Download protocol |
| IHC protocol for IDE antibody 67106-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging Cell Microglia-derived Galectin-9 drives amyloid-β pathology in Alzheimer's disease | ||
Mol Nutr Food Res Long-Term High-Fat Diet Decreases Renal Insulin-Degrading Enzyme Expression and Function by Inhibiting the PPARγ Pathway | ||
Mol Med Inhibition of insulin degrading enzyme suppresses osteoclast hyperactivity via enhancing Nrf2-dependent antioxidant response in glucocorticoid-induced osteonecrosis of the femoral head | ||
Phytomedicine Osmundacetone ameliorates Alzheimer's-like pathologies by inhibiting β-amyloid fibrillation, oxidative damage and neuroinflammation in APP/PS1 transgenic mice | ||
Phytomedicine Jiawei Xionggui Decoction promotes meningeal lymphatic vessels clearance of β-amyloid by inhibiting arachidonic acid pathway |
