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验证数据展示

  • FC experiment of BALB/c spleen cells using APC-FcA98341

    FC experiment of BALB/c spleen cells using APC-FcA98341

    1x10^6 untreated (left) or anti-CD3/CD28 treated (right) BALB/c spleen cells were surface stained with FcZero-rAb™ PE Anti-Mouse CD3e (145-2C11) and 0.1 ug APC Anti-Mouse ICOS/CD278 Rabbit RecAb (APC-FcA98341, Clone:242639C3). Cells were not fixed.

  • FC experiment of BALB/c spleen cells using APC-FcA98341

    FC experiment of BALB/c spleen cells using APC-FcA98341

    1x10^6 anti-CD3/CD28 treated BALB/c spleen cells were surface stained with 0.1 ug APC Anti-Mouse ICOS/CD278 Rabbit RecAb (APC-FcA98341, Clone: 242639C3) (red) or FcZero-rAb™ APC Rabbit IgG Isotype Control Recombinant Antibody (APC-FcA98136, Clone: 240953C9) (blue). 1x10^6 untreated BALB/c spleen cells were surface stained with 0.1 ug APC Anti-Mouse ICOS/CD278 Rabbit RecAb (APC-FcA98341, Clone: 242639C3) (black, dashed). Cells were not fixed.

  • Zero Fc receptor binding
  • Site-specific conjugation
  • One universal isotype

FcZero-rAb® APC Anti-Mouse ICOS/CD278 Rabbit Recombinant Antibody

ICOS/CD278 FcZero-rAb® Recombinant Antibody for FC
Cat No. APC-FcA98341

产品说明书

CloneNo. 242639C3

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

Activation-inducible lymphocyte immunomediatory molecule, Ailim, CCLP, CD278, CD28 and CTLA-4-like protein

缓冲液配方:  PBS, Azide, BSA
PBS, Azide, BSA
偶联物:  APC
规格价格库存

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经过测试的应用

Positive FC detected inanti-CD3/CD28 treated BALB/c spleen cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.1 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-FcA98341 targets ICOS/CD278 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原

CatNo: Eg3198

Product name: Recombinant Mouse ICOS/CD278 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 21-144 aa of NM_017480.2

Sequence: EINGSADHRMFSFHNGGVQISCKYPETVQQLKMRLFREREVLCELTKTKGSGNAVSIKNPMLCLYHLSNNSVSFFLNNPDSSQGSYYFCSLSIFDPPPFQERNLSGGYLHIYESQLCCQLKLWL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 inducible T-cell co-stimulator
别名 Activation-inducible lymphocyte immunomediatory molecule, Ailim, CCLP, CD278, CD28 and CTLA-4-like protein
计算分子量23kDa
GenBank蛋白编号NM_017480.2
基因名称 Icos
Gene ID (NCBI) 54167
RRIDAB_3746633
偶联类型 APC Fluorescent Dye
最大激发/发射波长650 nm / 660 nm
激发激光Red Laser (633 nm)
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9WVS0
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Inducible T cell co-stimulator (ICOS) is related to the CD28 superfamily and is highly expressed on activated T cells as well as regulatory T cells and is crucial for the survival and function of T cells, Th2 cell differentiation, and lung inflammatory responses. Binding ICOS to ICOS-ligand (ICOS-L) activates a cascade of intracellular signaling molecules that prevent apoptosis and lead to the production of cytokines such as IL-4 and IL-13.

实验方案

Product Specific Protocols
FC protocol for APC ICOS/CD278 antibody APC-FcA98341Download protocol
Standard Protocols
Click here to view our Standard Protocols
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