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验证数据展示

  • WB analysis using 67014-1-Ig (same clone as 67014-1-PBS)

    WB analysis using 67014-1-Ig (same clone as 67014-1-PBS)

    Different amount of human blood lysates were subjected to SDS PAGE followed by western blot with 67014-1-Ig (ICAM4 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • IHC staining of human spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    IHC staining of human spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ICAM4 antibody included
  • IHC staining of human spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    IHC staining of human spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ICAM4 antibody included
  • IF Staining of mouse spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    IF Staining of mouse spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ICAM4 antibody (67014-1-Ig, Clone: 3A9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    IF Staining of mouse spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ICAM4 antibody (67014-1-Ig, Clone: 3A9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

ICAM4 Monoclonal antibody, PBS Only

ICAM4 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67014-1-PBS

产品说明书

CloneNo. 3A9B9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

CD242, ICAM 4, ICAM-4, Landsteiner-Wiener blood group glycoprotein, LW

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ICAM4 Ready-To-Use IHC Kit. ICAM4 primary antibody included.

产品信息

67014-1-PBS targets ICAM4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag27628

Product name: Recombinant human ICAM4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 23-152 aa of BC029364

Sequence: ALGRRTKRAQSPKGSPLAPSGTSVPFWVRMSPEFVAVQPGKSVQLNCSNSCPQPQNSSLRTPLRQGKTLRGPGWVSYQLLDVRAWSSLAHCLVTCAGKTRWATSRITAYKPPHSVILEPPVLKGRKYTLR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)
别名 CD242, ICAM 4, ICAM-4, Landsteiner-Wiener blood group glycoprotein, LW
计算分子量 271 aa, 29 kDa
观测分子量 42 kDa
GenBank蛋白编号BC029364
基因名称 ICAM4
Gene ID (NCBI) 3386
RRIDAB_2882330
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14773
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

This gene encodes the Landsteiner-Wiener (LW) blood group antigen(s) that belongs to the immunoglobulin (Ig) superfamily, and that shares similarity with the intercellular adhesion molecule (ICAM) protein family. The protein migrate as a 42 kDa glycoprotein on SDS PAGE. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

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