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Species Reactivity
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 98011-4-RR (same clone as 98011-4-PBS)

    FC experiment of human PBMCs using 98011-4-RR (same clone as 98011-4-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ICAM-1/CD54 Rabbit Recombinant Antibody (98011-4-RR, Clone: 240646A11) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98011-4-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98011-4-RR (same clone as 98011-4-PBS)

    FC experiment of human PBMCs using 98011-4-RR (same clone as 98011-4-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ICAM-1/CD54 Rabbit Recombinant Antibody (98011-4-RR, Clone:240646A11)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98011-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98011-4-RR

    Affinity and Kinetic Characterization of 98011-4-RR

    Biolayer interferometry (BLl) kinetic assays of 98011-4-RR against Human ICAM-1/CD54 were performed. The affinity constant is 2.15 nM.

Anti-Human ICAM-1/CD54 Rabbit Recombinant Antibody, PBS Only

ICAM-1/CD54 Uni-rAb® Recombinant Antibody for FC
Cat No. 98011-4-PBS

产品说明书

CloneNo. 240646A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CD54, ICAM-1, ICAM1, ICAM 1, BB2

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ICAM-1 Ready-To-Use IHC Kit. ICAM-1 primary antibody included.

产品信息

98011-4-PBS targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 intercellular adhesion molecule 1
别名 CD54, ICAM-1, ICAM1, ICAM 1, BB2
计算分子量 90 kDa
GenBank蛋白编号BC015969
基因名称 ICAM-1
Gene ID (NCBI) 3383
ENSEMBL Gene IDENSG00000090339
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP05362
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

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