验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, U2OS cells |
| Positive IP detected in | U2OS cells |
| Positive IHC detected in | human testis tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26942-1-AP targets IARS in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | IARS fusion protein Ag25686 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | isoleucyl-tRNA synthetase |
| 别名 | IARS, IARS1, IleRS, ILRS, IRS, Isoleucine tRNA ligase, isoleucyl tRNA synthetase, PRO0785 |
| 计算分子量 | 144 kDa |
| 观测分子量 | 145 kDa |
| GenBank蛋白编号 | BC065552 |
| 基因名称 | IARS |
| Gene ID (NCBI) | 3376 |
| RRID | AB_2880693 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P41252 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IARS antibody 26942-1-AP | Download protocol |
| IHC protocol for IARS antibody 26942-1-AP | Download protocol |
| IP protocol for IARS antibody 26942-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation | ||
Food Chem Toxicol The crucial role of thioredoxin interacting protein in the liver insulin resistance induced by di (2-ethylhexyl) phthalates. | ||
Sci Rep Intermittent fasting activates macrophage migration inhibitory factor and alleviates high-fat diet-induced nonalcoholic fatty liver disease | ||
Discov Oncol High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer | ||
Nat Commun Time-resolved proteomic profiling reveals compositional and functional transitions across the stress granule life cycle | ||
Proc Natl Acad Sci U S A Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex
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