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IARS Polyclonal antibody

IARS Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 26942-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IP, IHC, ELISA and More (1)

IARS, IARS1, IleRS, ILRS, IRS, Isoleucine tRNA ligase, isoleucyl tRNA synthetase, PRO0785

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inK-562 cells, U2OS cells
Positive IP detected inU2OS cells
Positive IHC detected inhuman testis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26942-1-AP targets IARS in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原 IARS fusion protein Ag25686 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 isoleucyl-tRNA synthetase
别名 IARS, IARS1, IleRS, ILRS, IRS, Isoleucine tRNA ligase, isoleucyl tRNA synthetase, PRO0785
计算分子量 144 kDa
观测分子量 145 kDa
GenBank蛋白编号BC065552
基因名称 IARS
Gene ID (NCBI) 3376
RRIDAB_2880693
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP41252
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IARS antibody 26942-1-APDownload protocol
IHC protocol for IARS antibody 26942-1-APDownload protocol
IP protocol for IARS antibody 26942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Food Chem Toxicol

The crucial role of thioredoxin interacting protein in the liver insulin resistance induced by di (2-ethylhexyl) phthalates.

Authors - Wang Zhang
mouseWB

Sci Rep

Intermittent fasting activates macrophage migration inhibitory factor and alleviates high-fat diet-induced nonalcoholic fatty liver disease

Authors - Dezhao Li
humanWB,IF

Discov Oncol

High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer

Authors - Haiqing Wang
humanIF

Nat Commun

Time-resolved proteomic profiling reveals compositional and functional transitions across the stress granule life cycle

Authors - Shuyao Hu
humanWB

Proc Natl Acad Sci U S A

Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex

Authors - Krishnendu Khan
  • KD Validated
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