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验证数据展示

  • IF Staining of HepG2 using CL594-66045

    IF Staining of HepG2 using CL594-66045

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66045 (IA-2/PTPRN antibody) at dilution of 1:100.

  • FC experiment of HEK-293 using CL594-66045

    FC experiment of HEK-293 using CL594-66045

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human IA-2/PTPRN (CL594-66045, Clone:1D3F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • FC experiment of Y79 using CL594-66045

    FC experiment of Y79 using CL594-66045

    1X10^6 Y79 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human IA-2/PTPRN (CL594-66045, Clone:1D3F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite®594-conjugated IA-2/PTPRN Monoclonal antibody

IA-2/PTPRN Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66045

产品说明书

CloneNo. 1D3F5

宿主/亚型

Mouse / IgG3

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

IA-2, PTPRN, 1D3F5, ICA 512, ICA512-cleaved cytosolic fragment

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHEK-293 cells, Y79 cells
Planning an IHC experiment? We recommend our IHCeasy PTPRN Ready-To-Use IHC Kit. PTPRN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66045-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66045 targets IA-2/PTPRN in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag17909

Product name: Recombinant human PTPRN protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 510-811 aa of BC007713

Sequence: WCEEPAQANMDISTGHMILAYMEDHLRNRDRLAKEWQALCAYQAEPNTCATAQGEGNIKKNRHPDFLPYDHARIKLKVESSPSRSDYINASPIIEHDPRMPAYIATQGPLSHTIADFWQMVWESGCTVIVMLTPLVEDGVKQCDRYWPDEGASLYHVYEVNLVSEHIWCEDFLVRSFYLKNVQTQETRTLTQFHFLSWPAEGTPASTRPLLDFRRKVNKCYRGRSCPIIVHCSDGAGRTGTYILIDMVLNRMAKGVKEIDIAATLEHVRDQRPGLVRSKDQFEFALTAVAEEVNAILKALPQ

种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, N
别名 IA-2, PTPRN, 1D3F5, ICA 512, ICA512-cleaved cytosolic fragment
计算分子量 105 kDa
观测分子量 60 kDa
GenBank蛋白编号BC007713
基因名称 IA-2/PTPRN
Gene ID (NCBI) 5798
RRIDAB_2883490
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16849
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.

实验方案

Product Specific Protocols
FC protocol for CL594 IA-2/PTPRN antibody CL594-66045Download protocol
IF protocol for CL594 IA-2/PTPRN antibody CL594-66045Download protocol
Standard Protocols
Click here to view our Standard Protocols
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