Speedy™ Human/Cynomolgus Monkey TNF-alpha One-Step ELISA Kit
Sensitivity
1.1 pg/mL
Range
3.9-250 pg/mL
Reactivity
Human/Cynomolgus Monkey
Validation Data Gallery
Product Information
| Product name | Speedy™ Human/Cynomolgus Monkey TNF-alpha One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 3.9-250 pg/mL |
| Reactivity | Human/Cynomolgus Monkey |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7124 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 92% | 76%-99% |
| Cell culture supernatant | 107% | 100%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 128.6 | 7.6 | 5.9 |
| 2 | 8 | 30.3 | 0.8 | 2.8 |
| 3 | 8 | 15.1 | 0.4 | 2.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 127.7 | 6.2 | 4.9 |
| 2 | 16 | 30.5 | 1.0 | 3.3 |
| 3 | 16 | 15.2 | 0.5 | 3.4 |
Background Information
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression and inflammatory bowel disease (IBD).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Adiponectin, ADIPOQ, TNF, TNF alpha, TNF-a |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
