Speedy™ Human TNF-alpha One-Step ELISA Kit
Sensitivity
1.1 pg/mL
Range
3.9-250 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
| Product name | Speedy™ Human TNF-alpha One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 3.9-250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7124 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 92% | 76%-99% |
| Cell culture supernatant | 107% | 100%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 128.6 | 7.6 | 5.9 |
| 2 | 8 | 30.3 | 0.8 | 2.8 |
| 3 | 8 | 15.1 | 0.4 | 2.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 127.7 | 6.2 | 4.9 |
| 2 | 16 | 30.5 | 1.0 | 3.3 |
| 3 | 16 | 15.2 | 0.5 | 3.4 |
Background Information
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression and inflammatory bowel disease (IBD).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Adiponectin, ADIPOQ, TNF, TNF alpha, TNF-a |
