Human Total IL-18 ELISA Kit
Cat no : KE00025
Synonyms
Iboctadekin, IGIF, IL 1 gamma, IL 18, IL 1g, IL18, IL-18, IL1F4, Interleukin 1 gamma, Interleukin 18, Pre-IL18
Validation Data Gallery
Product Information
KE00025 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL18 ELISA kit is to be used to detect and quantify protein levels of endogenous Pre-IL18 and mature IL18. The assay recognizes human IL18. An antibody specific for IL18 has been pre-coated onto the microwells. The IL18 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL18 is added to detect the captured IL18 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
| Product name | Human Total IL-18 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3606 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 87% | 77%-110% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 512.7 | 18.4 | 3.6 |
| 2 | 20 | 265.8 | 9.9 | 3.7 |
| 3 | 20 | 89.9 | 5.5 | 6.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 495.5 | 20.6 | 4.2 |
| 2 | 24 | 258.4 | 13.3 | 5.2 |
| 3 | 24 | 90.9 | 4.2 | 4.7 |
Background Information
IL-18 is a member of IL-1 superfamily of cytokines. IL-18 is expressed by many human lymphoid and nonlymphoid cells, and has an important role in inflammatory processes. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL-18 synergistically with IL-12 contributes to Th1 differentiation and, therefore, is important in host defense mechanisms against intracellular bacteria, viruses, and fungi. The IL-18 cytokine increased expression of vascular cell adhesion molecule-1(VCAM1) and the adherence of melanoma cells. IL-18 plays a major role in atherosclerotic plaque destabilization leading to acute ischemic syndromes. IL-18 negatively regulate NAFLD/NASH progression, as well as multiple aspects of metabolic syndrome via modulation of the gut microbiota. This kit is used to quantify Pre-IL18 and mature IL18 level in vivo.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Iboctadekin, IGIF, IL 1 gamma, IL 18, IL 1g, IL18, IL-18, IL1F4, Interleukin 1 gamma, Interleukin 18, Pre-IL18 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Cell Death Dis N6-methyladenosine modification of circCUX1 confers radioresistance of hypopharyngeal squamous cell carcinoma through caspase1 pathway. | ||
Biomedicines Ruscogenin Attenuates Ulcerative Colitis in Mice by Inhibiting Caspase-1-Dependent Pyroptosis via the TLR4/NF-κB Signaling Pathway | ||
Heliyon Conditional reprogrammed human limbal epithelial cell model for anti-SARS-CoV-2 drug screening | ||
J Neuroinflammation Glutamine metabolism modulates microglial NLRP3 inflammasome activity through mitophagy in Alzheimer's disease |
