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CoraLite® Plus 488-conjugated IgG Polyclonal antibody

Human IgG Polyclonal Antibody for IF-P, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P, WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-10284

Print datasheet

Synonyms

Human IgG

验证数据展示

  • IF Staining of human tonsillitis using CL488-10284

    IF Staining of human tonsillitis using CL488-10284

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 Human IgG antibody (CL488-10284) at dilution of 1:200.

  • WB analysis using CL488-10284

    WB analysis using CL488-10284

    Human plasma were subjected to SDS PAGE followed by western blot with CL488-10284 (Human IgG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman plasma
Positive IF detected inhuman tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 10284-1-AP

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-10284 targets Human IgG in WB, IF applications and shows reactivity with human samples.

Tested Applications IF-P, WB
Cited ApplicationsIF
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Human IgG
Synonyms Human IgG
Calculated Molecular Weight 155 kDa
Observed Molecular Weight 25 kDa, 50 kDa, 150 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2918970
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This rabbit poly clonal antibody recognizes the human IgG.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 Human IgG antibody CL488-10284Download protocol
IF protocol for CL Plus 488 Human IgG antibody CL488-10284Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Oxid Med Cell Longev

Adropin Improves Radiation-Induced Myocardial Injury via VEGFR2/PI3K/Akt Pathway

Authors - Bingda Li
View Article