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验证数据展示

  • WB analysis of human milk using 87606-3-RR

    WB analysis of human milk using 87606-3-RR

    human milk were subjected to SDS PAGE followed by western blot with 87606-3-RR (Human IgA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 87606-3-RR

    IHC staining of human tonsillitis using 87606-3-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87606-3-RR (Human IgA1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Human IgA antibody included
  • IF Staining of human tonsillitis using 87606-3-RR

    IF Staining of human tonsillitis using 87606-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using Human IgA1 antibody (87606-3-RR, Clone: 252592A11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Human IgA1 Recombinant monoclonal antibody

Human IgA1 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 87606-3-RR

产品说明书

CloneNo. 252592A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

Human IgA, Human IgA Heavy Chain, IGHA1, IgA, IgA1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman milk
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy Human IgA Ready-To-Use IHC Kit. Human IgA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87606-3-RR targets Human IgA1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg9190

Product name: Recombinant human IgA1-IgK protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 10011193 of

Sequence: \

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 immunoglobulin heavy constant alpha 1
别名 Human IgA, Human IgA Heavy Chain, IGHA1, IgA, IgA1
观测分子量50-70 kDa
基因名称 Human IgA Heavy Chain
Gene ID (NCBI) 3493
RRIDAB_3745646
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human IgA1 is one of the two subclasses of Immunoglobulin A (IgA), the most abundantly produced antibody in the human body . While IgA is the predominant antibody class at mucosal surfaces (such as the gut, respiratory, and urogenital tracts), IgA1 is the dominant subclass in the blood (serum), comprising approximately 85-90% of total serum IgA .

实验方案

Product Specific Protocols
IF protocol for Human IgA1 antibody 87606-3-RRDownload protocol
IHC protocol for Human IgA1 antibody 87606-3-RRDownload protocol
WB protocol for Human IgA1 antibody 87606-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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