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验证数据展示

  • WB analysis of Recombinant protein using 87680-2-RR

    WB analysis of Recombinant protein using 87680-2-RR

    Recombinant protein were subjected to SDS PAGE followed by western blot with 87680-2-RR (Human Ig, λ Light Chain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human plasma using 87680-2-RR

    WB analysis of human plasma using 87680-2-RR

    human plasma were subjected to SDS PAGE followed by western blot with 87680-2-RR (Human Ig, λ Light Chain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 87680-2-RR

    IHC staining of human tonsillitis using 87680-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87680-2-RR (Human Ig, λ Light Chain 1 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 87680-2-RR

    IHC staining of human tonsillitis using 87680-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87286-17-RR (Human Ig, λ Light Chain 1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Human Ig Lambda Light Chain Recombinant monoclonal antibody

Human Ig Lambda Light Chain Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 87680-2-RR

产品说明书

CloneNo. 251292B12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Ig Lambda Light Chain, Ig Lambda, Ig λ Light Chain, IgG1, IGHG 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma, Recombinant protein
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87680-2-RR targets Human Ig Lambda Light Chain in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg6676

Product name: recombinant human IgG1-IgL1 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 10009502 of

Sequence: /

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 immunoglobulin heavy constant gamma 1 (G1m marker)
别名 Ig Lambda Light Chain, Ig Lambda, Ig λ Light Chain, IgG1, IGHG 1
观测分子量25-30 kDa
基因名称 IGHG1
Gene ID (NCBI) 3500
RRIDAB_3745694
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human Immunoglobulin Lambda Light Chain 1, encoded by the IGLC1 gene, is the constant region of the lambda light chain that pairs with a variable region to form a functional antibody, playing a critical role in immune response by contributing to antigen binding and structural diversity.

实验方案

Product Specific Protocols
IHC protocol for Human Ig Lambda Light Chain antibody 87680-2-RRDownload protocol
WB protocol for Human Ig Lambda Light Chain antibody 87680-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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