Human HSP60 ELISA Kit
Cat no : KE00032
Synonyms
60 kDa chaperonin, GROEL, Heat shock protein 60, HLD4, HSP60, HSP65, HuCHA60, P60 lymphocyte protein, SPG13, HSPD1, HSP 60, CPN60, Chaperonin 60
Validation Data Gallery
Product Information
KE00032 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HSPD1 ELISA kit is to be used to detect and quantify protein levels of endogenous HSPD1. The assay recognizes human HSPD1. A polyclonal antibody specific for HSPD1 has been pre-coated onto the microwells. The HSPD1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for HSPD1 is added to detect the captured HSPD1 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
| Product name | Human HSP60 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Plasma, Serum 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 7 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3329 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Plasma | 99% | 86%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
Background Information
HSPD1(also known as HSP60) belongs to the chaperonin family and acts as a chaperone essential for protein folding. HSP60 is a widely expressed protein and has been connected with many aspects of cell functions including gene expression regulation, DNA replication, signal transduction, differentiation, apoptosis, and cellular senescence or immortalisation. Recently it has been reported that HSP60 was significantly up-regulated in squamous cell lung cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 60 kDa chaperonin, GROEL, Heat shock protein 60, HLD4, HSP60, HSP65, HuCHA60, P60 lymphocyte protein, SPG13, HSPD1, HSP 60, CPN60, Chaperonin 60 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Anal Methods Chem Developing a Novel Indolocarbazole as Histone Deacetylases Inhibitor against Leukemia Cell Lines. | ||
Curr Issues Mol Biol Bioactive Component Screening and Mechanistic Study of the Anti-Diabetic Activity of Lophatherum gracile Brongn Extract |
