Human GKN1 ELISA Kit
Sensitivity
5 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat no : KE00029
Synonyms
18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11
Validation Data Gallery
Product Information
KE00029 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GKN1 ELISA kit is to be used to detect and quantify protein levels of endogenous GKN1. The assay recognizes human GKN1. A polyclonal antibody specific for GKN1 has been pre-coated onto the microwells. The GKN1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for GKN1 is added to detect the captured GKN1 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
| Product name | Human GKN1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Plasma, Serum 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 5 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 56287 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Plasma | 95% | 85%-121% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
Background Information
Gastrokine 1 (GKN1), a stomach-speci?c protein also known as 18 kDa antrum mucosa protein (AMP-18) or foveolin, belongs to the gastrokine family of gastric mucus cell-secreted proteins. The human GKN1 gene is localized in a region of chromosome 2p13 of about 6 kb and contains 6 exons. As a secreted protein, GKN1 is specifically expressed in gastric mucosa to protect and maintain the integrity of gastric epithelium. GKN1 is involved in the replenishment of the surface lumen epithelial cell layer, and could play a role in cell proliferation and differentiation. Recent findings showed that GKN1 may act as a functional gastric tumor suppressor and may also modulate apoptotic signals.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
