Speedy™ Human Endoglin/CD105 One-Step ELISA Kit
Sensitivity
6.6 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Endoglin/CD105 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 6.6 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2022 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 108% | 92%-120% |
| Cell culture supernatant | 101% | 94%-109% |
| Lysate | 97% | 91%-106% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,054.7 | 45.0 | 4.3 |
| 2 | 8 | 268.1 | 6.9 | 2.6 |
| 3 | 8 | 136.4 | 4.3 | 3.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,035.1 | 40.3 | 3.9 |
| 2 | 16 | 262.2 | 9.1 | 3.5 |
| 3 | 16 | 133.5 | 4.5 | 3.4 |
Background Information
Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. The extracellular domain of membrane-bound Endoglin can be proteolytically cleaved, releasing a soluble form of Endoglin (sCD105). Increased levels of sCD105 are linked to the pathogenesis of severe vascular disease, and also correlate with poor prognosis in patients suffering from various types of cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD105, END, Endoglin, ENG, HHT1 |
FAQ
1、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
