Recombinant human CPE protein (rFc Tag)
种属
Human
纯度
>85 %, SDS-PAGE
标签
C-rFc
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >85 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human CPE protein Glu26-Ser453 (Accession# P16870-1) with a rabbit IgG Fc tag at the C-terminus |
| 基因ID | 1363 |
| 蛋白编号 | P16870-1 |
| 预测分子量 | 73.9 kDa |
| SDS-PAGE | 68-80 kDa, reducing (R) condition |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
CPE is a multifunctional protein harboring both enzymatic and nonenzymatic functions, largely expressed in endocrine cells and peptidergic neurons in the nervous system. Full-length CPE (F-CPE) protein is an inhibitor of Wnt/β-catenin signaling, which disrupts disheveled-induced signalosomes that are important for transducing the Wnt signal and reduces β-catenin protein levels and activity (PMID: 22824791). Defects in the gene encoding carboxypeptidase E (CPE) in either mouse or human lead to multiple endocrine disorders, including obesity and diabetes (PMID: 16219026).
参考文献:
1. Skalka N. et al. (2013). Oncogene. 32(23):2836-47. 2. Zhu X. et al. (2005). J Neurochem. 95(5):1351-62.